Human IL-21 ELISA Kit (ab119542)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-21 ELISA Kit
IL-21 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 8 5.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 8 7.7% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
20 pg/ml -
検出範囲
78 pg/ml - 5000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 95 % - % Serum 71 % - % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-21 concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).
IL-21 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-21 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-21 captured on the plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-human IL-21 monoclonal antibody 1 x 70µl Human IL-21 Standard lyophilized (10 ng/ml upon reconstitution) 2 vials Microplate coated with monoclonal antibody to Human IL-21 1 unit Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究分野
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関連性
A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. IL-21 has been found to be a powerful growth factor for naive B cells. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAKSTAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. -
細胞内局在
Secreted -
別名
- Cytokine CX2
- DN 452A22.7
- IL 21
see all -
参照データベース
- Entrez Gene: 59067 Human
- Omim: 605384 Human
- SwissProt: Q9HBE4 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab119542 は 5 報の論文で使用されています。
- Zhang Y et al. The Regulation between CD4+CXCR5+ Follicular Helper T (Tfh) Cells and CD19+CD24hiCD38hi Regulatory B (Breg) Cells in Gastric Cancer. J Immunol Res 2022:9003902 (2022). PubMed: 36339942
- Wang X et al. MicroRNA-146a-5p enhances T helper 17 cell differentiation via decreasing a disintegrin and metalloprotease 17 level in primary sjögren's syndrome. Bioengineered 12:310-324 (2021). PubMed: 33446013
- Liu Z et al. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci 17:3065-3072 (2020). PubMed: 33173427
- Yamamoto M et al. Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment. Mod Rheumatol N/A:1-5 (2018). PubMed: 29385874
- Zhang Z et al. Astragalus mongholicus (Fisch.) Bge Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155. Front Pediatr 6:139 (2018). PubMed: 29977885