Human IL-2 Receptor ELISA Kit (Interleukin-2 Receptor) (ab46036)

製品の概要

  • 製品名
    Human IL-2 Receptor ELISA Kit (Interleukin-2 Receptor)
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    1 6 1594pg/ml 184 11.6%
    2 6 1528pg/ml 135 8.8%
    3 6 1705pg/ml 204 12%
    4 6 1124pg/ml 171 15.2%
    5 6 2305pg/ml 165 7.2%
    6 6 1824pg/ml 126 69%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    32.5 pg/ml
  • 検出範囲
    68.75 pg/ml - 2220 pg/ml
  • 添加回収試験

    87 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 99 86% - 122%

  • 全工程の試験時間
    3h 40m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-2 Receptor (IL-2R) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2R in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant Human IL-2R.

    A monoclonal antibody specific for IL-2R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2R concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2R are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2R present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-2 Receptor.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-IL-2R Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-2R Control Silver 2 vials 4 vials
    IL-2R Microplate (12 x 8 well strips) 1 unit 2 units
    Plastic Plate Covers 2 units 4 units
    Stop Reagent Black 1 x 11ml 1 x 11ml
    Streptavidin-HRP 2 vials 4 vials
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 関連性
    The IL2 Receptor, which is involved in T cell-mediated immune responses, alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Homodimeric beta (IL2RB) chains produce a medium-affinity receptor. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The interleukin 2 (IL2) receptor gamma chain (IL2RG), an important signalling component of many interleukin receptors (IL2,IL4,IL7,IL9, and IL15), is thus referred to as the common gamma chain. Mutations in this X-chromosome-linked gene cause X-linked severe combined immunodeficiency (XSCID).
  • 細胞内局在
    Cell Membrane; single-pass type I membrane protein.
  • 別名
    • CD122
    • CD122 antigen
    • CD132
    • CD132 antigen
    • CD25
    • CD25 antigen
    • IL2RA
    • IL2RB
    • IL2RG
    • IMD4
    • interleukin 2 receptor alpha
    • interleukin 2 receptor beta
    • interleukin 2 receptor gamma
    • p55
    • p64
    • p75
    • SCIDX
    • SCIDX1
    • TAC antigen
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab46036

プロトコール

参考文献

This product has been referenced in:
  • Ngcobo M & Gqaleni N Evaluation of the immunomodulatory effects of a South African commercial traditional immune booster in human peripheral blood mononuclear cells. BMC Complement Altern Med 16:300 (2016). Read more (PubMed: 27550057) »

See 1 Publication for this product

レビューと Q&A

Thank you for contacting us. The two soluble IL2 receptor ELISA kits, ab46036 (with plate) and ab47354 (capture and detection antibodies sufficient for five plates, but plates are not included) are known to be reactive with the alpha sub-unit. Reactivi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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