Human IL-2 ELISA Kit (Interleukin-2) High Sensitivity (ab46054)

製品の概要

  • 製品名
    Human IL-2 ELISA Kit (Interleukin-2) High Sensitivity
    IL2 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5 4.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 5 9%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 0.97 pg/ml
  • 検出範囲
    1.87 pg/ml - 60 pg/ml
  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95 % - %
    Serum 100 % - %

  • 全工程の試験時間
    2h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human High Sensitivity IL-2 ELISA is to be used for the in-vitro quantitative determination interleukin-2 (IL-2) in Human sera, plasmas, buffered solutions or cell culture media.


    A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-2.


    Get results in 90 minutes with Human IL-2 ELISA Kit (ab174444) from our SimpleStep ELISA® range.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml
    200X Wash Buffer White 1 x 10ml
    Amplification Diluent Brown & blue spot 1 x 25ml
    Amplifier Yellow 1 x 200µl
    Biotinylated anti IL-2 Red 1 x 400µl
    Biotinylated Antibody Diluent Red 1 x 7.5ml
    Chromogen TMB Substrate Solution 1 x 11ml
    HRP Diluent Red 1 x 23ml
    IL-2 Microplate (12 x 8 well strips) 1 unit
    IL-2 Standard (Lyophilized) Yellow 2 vials
    Standard Diluent (Human Serum) 1 x 7ml
    Stop Reagent Black 1 x 11ml
    Streptavidin-HRP 2 x 5µl
  • 研究分野
  • 機能
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 関連疾患
    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • 配列類似性
    Belongs to the IL-2 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab46054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab46054

プロトコール

参考文献

ab46054 has not yet been referenced specifically in any publications.

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