Human IL-1 alpha ELISA Kit (ab100560)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 0.82 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-1 alpha ELISA Kit
IL-1 alpha キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
0.82 pg/ml - 600 pg/ml -
添加回収試験
95 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 102.25 89% - 108% Serum 91.89 80% - 101% Plasma 92.46 82% - 102% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-1 alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human IL-1a ELISA Kit (ab178008) from our SimpleStep ELISA® range.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-1 alpha 2 vials IL-1 alpha Microplate (12 x 8 wells) 1 unit Recombinant Human IL-1 alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性
IL-1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. -
細胞内局在
Secreted -
別名
- Hematopoietin 1
- IL 1
- IL 1 alpha
see all -
参照データベース
- Entrez Gene: 3552 Human
- Omim: 147760 Human
- SwissProt: P01583 Human
関連製品
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SimpleStep ELISA kits
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab100560 は 1 報の論文で使用されています。
- Yang H et al. Ovarian IL-1a and IL-1ß levels are associated with primary ovarian insufficiency. Int J Clin Exp Pathol 11:4711-4717 (2018). PubMed: 31949872