Human IL-18 BP ELISA Kit (Interleukin-18 Binding Protein) (ab100559)

製品の概要

  • 製品名
    Human IL-18 BP ELISA Kit (Interleukin-18 Binding Protein)
    IL18 binding protein キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    24.69 pg/ml - 18000 pg/ml
  • 添加回収試験

    91 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 106.7 96% - 121%
    Serum 83.5 74% - 97%
    Plasma 82.3 72% - 96%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-18 BP (Interleukin-18 Binding Protein) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 BP in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-18 BP coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18 BP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 BP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 BP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab100559

  • Representative standard curve using ab100559

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参考文献

This product has been referenced in:
  • Menale C  et al. Bisphenol A effects on gene expression in adipocytes from children: association with metabolic disorders. J Mol Endocrinol 54:289-303 (2015). Read more (PubMed: 25878060) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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