製品の概要

  • 製品名
    Human IL-17F ELISA Kit (Interleukin-17F)
    IL17F キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 8 7.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8 6.5%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    15.5 pg/ml
  • 検出範囲
    31.3 pg/ml - 2000 pg/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-17F (Interleukin-17) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-17F (Interleukin-17)concentrations in Cell culture supernatant, serum and plasma (citrate, heparin).

    IL-17F specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an Biotin-conjugated anti-Human IL-17F detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-17F captured on the plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119537.

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参考文献

ab119537 has not yet been referenced specifically in any publications.

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