製品の概要

  • 製品名
    Human IL-17A/F ELISA Kit (without plates)
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 9 230.3pg/ml 8.1 3.5%
    B 9 313.4pg/ml 19.2 6.1%
    C 9 435.5pg/ml 32.4 7.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 9 230.1pg/ml 9.7 4.2%
    B 9 313.2pg/ml 20.1 6.4%
    C 9 435.3pg/ml 31.3 7.2%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 7 pg/ml
  • 検出範囲
    15.6 pg/ml - 500 pg/ml
  • 添加回収試験

    107 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 102 77% - 117%

  • 全工程の試験時間
    3h 45m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-17A + IL-17F Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17A + IL-17F in supernatants, buffered solutions or serum and plasma samples and other body fluids.

    A monoclonal antibody specific for IL-17A + IL-17F has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17A + IL-17F concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-17A + IL-17F are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-17A + IL-17F present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-17A + IL-17F.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated Secondary Antibody 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-17A + IL-17F Microplate (12 x 8 well strips) 1 unit 2 units
    IL-17A/F standard (Lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 関連性
    IL17A induces stromal cells to produce proinflammatory and hematopoietic cytokines and enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. Il17F stimulates the production of other cytokines such as IL6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. It also stimulates PBMC and T-cell proliferation and inhibits angiogenesis.
  • 細胞内局在
    Secreted
  • 別名
    • C87042
    • CTLA8
    • Cytokine ML 1
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein
    • Cytotoxic T lymphocyte associated serine esterase 8
    • IL 17
    • IL 17A
    • IL 17F
    • IL 24
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • Interleukin 17F
    • Interleukin 24
    • MGC114402
    • ML 1
    • ML1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab105137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab105137

プロトコール

参考文献

ab105137 has not yet been referenced specifically in any publications.

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