Human IL-17A/F ELISA Kit (without plates) (ab105137)
製品の概要
-
製品名Human IL-17A/F ELISA Kit (without plates)
-
検出方法Colorimetric
-
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% A 9 230.3pg/ml 8.1 3.5% B 9 313.4pg/ml 19.2 6.1% C 9 435.5pg/ml 32.4 7.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% A 9 230.1pg/ml 9.7 4.2% B 9 313.2pg/ml 20.1 6.4% C 9 435.3pg/ml 31.3 7.2% -
サンプルの種類Cell culture supernatant, Serum, Plasma
-
アッセイタイプSandwich (quantitative)
-
検出感度< 7 pg/ml
-
検出範囲15.6 pg/ml - 500 pg/ml
-
添加回収試験
107 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 102 77% - 117% -
全工程の試験時間3h 45m
-
ステップMultiple steps standard assay
-
種交差性交差種: Human
-
製品の概要
Abcam’s IL-17A + IL-17F Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17A + IL-17F in supernatants, buffered solutions or serum and plasma samples and other body fluids.
A monoclonal antibody specific for IL-17A + IL-17F has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17A + IL-17F concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-17A + IL-17F are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-17A + IL-17F present in the samples.
This kit will recognize both endogenous and recombinant Human IL-17A + IL-17F.
-
アプリケーション適用あり: Sandwich ELISAmore details
-
試験プラットフォームMicroplate
法規制情報
製品の特性
-
保存方法Store at +4°C. Please refer to protocols.
-
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated Secondary Antibody 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 23ml IL-17A + IL-17F Microplate (12 x 8 well strips) 1 unit 2 units IL-17A/F standard (Lyophilized) Yellow 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
-
関連性IL17A induces stromal cells to produce proinflammatory and hematopoietic cytokines and enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. Il17F stimulates the production of other cytokines such as IL6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. It also stimulates PBMC and T-cell proliferation and inhibits angiogenesis.
-
細胞内局在Secreted
-
別名
- C87042
- CTLA8
- Cytokine ML 1
see all -
参照データベース
- Entrez Gene: 3605 Human
- Entrez Gene: 112744 Human
- Omim: 603149 Human
- Omim: 606496 Human
- SwissProt: Q16552 Human
- SwissProt: Q96PD4 Human
アプリケーション
Our Abpromise guarantee covers the use of ab105137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
-
Typical standard curveRepresentative standard curve using ab105137
プロトコール
参考文献
This product has been referenced in:
- Thiessen SE et al. The Role of Autophagy in Critical Illness-induced Liver Damage. Sci Rep 7:14150 (2017). Read more (PubMed: 29074879) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"