製品の概要

  • 製品名Human IL-17a ELISA Kit (Interleukin -17a)
    IL-17a キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 6 3.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 6 5.2%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 3 pg/ml
  • 検出範囲
    3.12 pg/ml - 100 pg/ml
  • 添加回収試験

    105 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 121 113% - 130%

  • 全工程の試験時間
    3h 45m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-17a (Interleukin-17a) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative of IL-17a in supernatants, buffered solutions, serum, plasma and other body fluids.


    A monoclonal antibody specific for IL-17a has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17a concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-17a are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-17a present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-17a.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.
  • 組織特異性Restricted to activated memory T-cells.
  • 配列類似性Belongs to the IL-17 family.
  • 翻訳後修飾Found both in glycosylated and nonglycosylated forms.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • CTLA8
    • IL17A
    • CTLA-8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin-17A
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab83688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-17a ELISA Kit (Interleukin -17a) 画像

  • IL-17 measured in cell culture supernatants (dilution range 1/1-1/100) from control (PBMC) or treated ((PBMC+PHA) 48 hours; 2% PHA-M; LifeTechnologies) cells (duplicates +/- SD).

  • Representative Standard Curve using ab83688

プロトコール

Human IL-17a ELISA Kit (Interleukin -17a) (ab83688) 使用論文

This product has been referenced in:
  • Yu X  et al. Intensive Cytokine induction in Pandemic H1N1 Influenza Virus Infection Accompanied by Robust Production of IL-10 and IL-6. PLoS One 6:e28680 (2011). ELISA . Read more (PubMed: 22174866) »

See 1 Publication for this product

Product Wall

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"