Human IL-17 ELISA Kit (Interleukin-17) (ab100556)

製品の概要

  • 製品名Human IL-17 ELISA Kit (Interleukin-17)
    IL17 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    15.63 pg/ml - 1000 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 93.65 84% - 103%
    Serum 94.15 82% - 103%
    Plasma 92.24 82% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-17 (Interleukin-17) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-17 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-17 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • 組織特異性Restricted to activated memory T-cells.
  • 配列類似性Belongs to the IL-17 family.
  • 翻訳後修飾Found both in glycosylated and nonglycosylated forms.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • CTLA 8
    • CTLA-8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17_HUMAN
    • Il17a
    • Interleukin 17A
    • Interleukin-17A
    • Interleukin17
    • Interleukin17A
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-17 ELISA Kit (Interleukin-17) 画像

  • Representative standard curve using ab100556

  • Representative standard curve using ab100556

プロトコール

Human IL-17 ELISA Kit (Interleukin-17) (ab100556) 使用論文

ab100556 has not yet been referenced specifically in any publications.

Product Wall

This kit recognizes IL-17A and does not cross react with IL-17B, C, D, E, or F. Whether the kit will also recognize the IL-17A/F heterodimer has not been tested but some degree of cross reactivity is possible based on sequence similarity.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"