製品の概要

  • 製品名Human IL-16 ELISA Kit (Interleukin-16)
    IL16 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    4.12 pg/ml - 3000 pg/ml
  • 添加回収試験

    104 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 107.5 97% - 117%
    Serum 113.7 103% - 122%
    Plasma 92.26 80% - 112%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-16 (Interleukin-16) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-16 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-16 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-16 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-16 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-16 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
    Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
    Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
  • 組織特異性Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.
  • 配列類似性Contains 4 PDZ (DHR) domains.
  • 翻訳後修飾Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
  • 細胞内局在Cytoplasm; Cytoplasm. Nucleus and Secreted.
  • Information by UniProt
  • 別名
    • FLJ16806
    • FLJ42735
    • FLJ44234
    • HGNC:5980
    • HsT19289
    • IL-16
    • IL16
    • IL16_HUMAN
    • Interleukin 16
    • Interleukin 16 precursor
    • Interleukin-16
    • KIAA4048
    • LCF
    • Lymphocyte chemoattractant factor
    • mKIAA4048
    • Neuronal interleukin 16
    • NIL16
    • OTTHUMP00000185571
    • OTTHUMP00000190849
    • OTTHUMP00000190850
    • PrIL16
    • Pro interleukin 16
    • Prointerleukin 16
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-16 ELISA Kit (Interleukin-16) 画像

  • Representative standard curve using ab100555

プロトコール

Human IL-16 ELISA Kit (Interleukin-16) (ab100555) 使用論文

ab100555 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab100555.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"