Human IL-15 ELISA Kit (Interleukin-15) (ab100554)

製品の概要

  • 製品名Human IL-15 ELISA Kit (Interleukin-15)
    IL15 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 3 pg/ml
  • 検出範囲
    3.3 pg/ml - 800 pg/ml
  • 添加回収試験

    94 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 96.37 83% - 104%
    Serum 92.26 80% - 102%
    Plasma 94.53 82% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-15 (Interleukin-15) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-15 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IL-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle.
  • 細胞内局在Isoform IL15-S48AA: Secreted. Isoform IL15-S21AA: Cytoplasm. Nucleus. Note: IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
  • 別名
    • IL 15
    • Interleukin 15
    • Interleukin15
    • MGC9721
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-15 ELISA Kit (Interleukin-15) 画像

  • Representative standard curve using ab100554

  • Representative standard curve using ab100554

プロトコール

Human IL-15 ELISA Kit (Interleukin-15) (ab100554) 使用論文

ab100554 has not yet been referenced specifically in any publications.

Product Wall

easy IL15 ELISA

Good Excellent 5/5 (Ease of Use)
Abreviews
IL15 was measured in human culture supernatants, diluted with assay diluent B as recommended.
Solid standard curve and very easy to use kit. However, according our ODs of the standards, the kit is not quite as sensitive as stated.
Username

Dr. Anna Vossenkaemper

Verified customer

投稿 Jun 30 2015

’ai contacté le laboratoire mais malheureusement comme expliqué par téléphone, nous n’avons pas testé ce kit avec Il-15 attaché au récepteur.

De plus, puisque nous ne connaissons pa...

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I am getting back to you in regards to the problems experienced when using this kit.

We have not yet encountered an issue with our ELISA kits that were caused by not printing the capture antibodies. All of our kits are pre-coated.

I...

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Thank you for contacting us.

I appreciate your patience and apologize for keeping you waiting.

As none of the standards gave strong signal responses it could be that the standard recombinant protein has degraded. However, because th...

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Thank you for contacting us.
I am very sorry to hear this kit failed to give the expected results. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
In order to better under...

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Thank you for contacting us.

Since the protocol recommends to store the concentrateat 4°C for 5 days, this is what would be best. If the concentrate is still good after 21 days and how good it is, that would need to be tried out and is d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"