製品の概要

  • 製品名Human IL-13 Receptor alpha ELISA Kit
    IL13R alpha2 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 14 3024pg/ml 93.67 3.1%
    B 14 1054pg/ml 29.69 2.82%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 8 2744pg/ml 90.1 3.28%
    B 8 725pg/ml 43.9 6.06%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 150 pg/ml
  • 検出範囲
    156 pg/ml - 5000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 90 156pg/ml - 5000pg/ml

  • 全工程の試験時間
    3h 45m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-13 Receptor alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interleukin 13 Receptor (IL-13 Receptor alpha, also known as CD213 alpha 2) in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-13 Receptor alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 Receptor alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 Receptor alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 Receptor alpha present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-13 Receptor alpha.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 関連性The Interleukin-13 receptor alpha-2 chain (IL3Ralpha2) belongs to the type I cytokine receptor family. It binds as a monomer with high affinity to interleukin 13, but lacks a cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13.
  • 細胞内局在Single-pass type I membrane protein
  • 別名
    • CD213A2
    • IL 13R
    • IL13BP
    • IL13R
    • Interleukin 13 binding protein
    • interleukin 13 receptor, alpha 2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-13 Receptor alpha ELISA Kit 画像

  • Representative Standard Curve using ab46066

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Human IL-13 Receptor alpha ELISA Kit (ab46066) 使用論文

ab46066 has not yet been referenced specifically in any publications.

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