Human IL-13 ELISA Kit (Interleukin-13) (ab46038)

製品の概要

  • 製品名
    Human IL-13 ELISA Kit (Interleukin-13)
    IL13 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 99.04pg/ml 0.476 0.48%
    Sample B 8 13.47pg/ml 0.423 3.14%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 58 99.95pg/ml 1.439 1.43%
    Sample B 58 13.27pg/ml 0.767 5.77%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 1.5 pg/ml
  • 検出範囲
    3.12 pg/ml - 100 pg/ml
  • 添加回収試験

    102.3 %

  • 全工程の試験時間
    2h 10m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-13 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-13 in Human serum, plasma, buffered solutions and cell culture media.


    A monoclonal antibody specific for IL-13 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 present in the samples. 


    This kit will recognize both endogenous and recombinant Human IL-13.


    Get results in 90 minutes with Human IL-13 ELISA Kit (ab178014) from our SimpleStep ELISA® range.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-13 Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-13 Control Silver 2 vials 4 vials
    IL-13 Microplate (12 x 8 well strips) 1 unit 2 units
    IL13 standard Yellow 2 vials 4 vials
    Standard Diluent (Human Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 機能
    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • 関連疾患
    Allergic rhinitis
  • 配列類似性
    Belongs to the IL-4/IL-13 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • Allergic rhinitis
    • ALRH
    • BHR1
    • Bronchial hyperresponsiveness 1 (bronchial asthma)
    • IL 13
    • IL-13
    • Il13
    • IL13_HUMAN
    • Interleukin 13
    • Interleukin-13
    • MGC116786
    • MGC116788
    • MGC116789
    • NC30
    • P600
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab46038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab46038

プロトコール

参考文献

ab46038 has not yet been referenced specifically in any publications.

レビューと Q&A

I received confirmation from the laboratory that samples/standards are co-incubate with the biotinylated Antibody (1h30 room temperature) in the IL-13 ELISA Kit. There's then a washing step and the incubation of the streptavidin-HRP as detailed in t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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