Human IL-13 ELISA Kit (ab100553)
Key features and details
- Sensitivity: 0.15 pg/ml
- Range: 0.16 pg/ml - 40 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human IL-13 ELISA Kit
IL-13 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.15 pg/ml -
検出範囲
0.16 pg/ml - 40 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 100.65 88% - 107% Serum 96.35 84% - 103% Plasma 94.39 82% - 102% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-13 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-13 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human IL-13 ELISA Kit (ab178014) from our SimpleStep ELISA® range.
-
特記事項
Optimization may be required with urine samples
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 120X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-13 2 vials IL-13 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-13 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
機能
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
関連疾患
Allergic rhinitis -
配列類似性
Belongs to the IL-4/IL-13 family. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- Allergic rhinitis
- ALRH
- BHR 1
see all -
参照データベース
- Entrez Gene: 3596 Human
- Omim: 147683 Human
- SwissProt: P35225 Human
- Unigene: 845 Human
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab100553 は 3 報の論文で使用されています。
- Ji Z et al. Neural stem cells induce M2 polarization of macrophages through the upregulation of interleukin-4. Exp Ther Med 20:148 (2020). PubMed: 33093886
- Yamamoto M et al. Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment. Mod Rheumatol N/A:1-5 (2018). PubMed: 29385874
- Hosseinkhani B et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). PubMed: 30131806