Human IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab100552)

製品の概要

• 製品名Human IL-12 p70 ELISA Kit (Interleukin-12 p70)
  IL12 p70 キット 製品一覧
• 検出方法Colorimetric
• サンプルの種類
  Cell culture supernatant, Plasma
• アッセイタイプSandwich (quantitative)
• 検出感度
  < 1 pg/ml
• 検出範囲
  0.8 pg/ml - 600 pg/ml
• 添加回収試験

  88 %
  

  特定サンプルでの回収試験

  サンプルの種類	平均 %	測定範囲
  Cell culture supernatant	95.52	88% - 109%
  Plasma	85.63	79% - 101%

• ステップMultiple steps standard assay
• 種交差性
  交差種: Human
  
• 製品の概要

  Abcam’s IL-12 p70 (Interleukin-12 p70) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 p70 in plasma and cell culture supernatants.

  
  

   This assay employs an antibody specific for Human IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  
  

  We have not been able to detect endogenous Human IL-12 p70 in normal serum with ab100552, only in serum spiked with Human IL-12 p70.

  
  

  Get results in 90 minutes with Human IL-12p40/p70 ELISA Kit (ab178009) from our SimpleStep ELISA^® range.

• 特記事項

  Optimization may be required with urine samples

• アプリケーション適用あり: Sandwich ELISAmore details
• 試験プラットフォームMicroplate

製品の特性

• 保存方法Store at -20°C. Please refer to protocols.

• 内容	1 x 96 tests
  20X Wash Buffer Concentrate	1 x 25ml
  300X HRP-Streptavidin Concentrate	1 x 200µl
  5X Assay Diluent B	1 x 15ml
  Assay Diluent A	1 x 30ml
  Biotinylated anti-Human IL-12 p70	2 vials
  IL-12 p70 Microplate (12 x 8 wells)	1 unit
  Recombinant IL-12 p70 Standard	2 vials
  Stop Solution	1 x 8ml
  TMB One-Step Substrate Reagent	1 x 12ml

• 研究分野
  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Interleukin ELISA kits

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Cytokines and cytokine receptors ELISA kits

Human IL-12 p70 ELISA Kit (Interleukin-12 p70) 画像

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  Typical standard curve

  Representative standard curve using ab100552

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  Typical standard curve

  Representative standard curve using ab100552