Human IL-12 + IL-23 p40 ELISA Kit (ab119534)
製品の概要
- 製品名Human IL-12 + IL-23 p40 ELISA Kit
- 検出方法Colorimetric
- 再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 8 4.7% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 8 6.4% - サンプルの種類Cell culture supernatant, Serum, Plasma
- アッセイタイプSandwich (quantitative)
- 検出感度< 10 pg/ml
- 検出範囲31.3 pg/ml - 2000 pg/ml
- 添加回収試験
71 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 71 65% - 75% - ステップMultiple steps standard assay
- 種交差性交差種: Human
- 製品の概要
Abcam’s IL-12 + IL-23 p40 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-12 + IL-23 p40 concentrations in Cell culture supernatant, serum and plasma (citrate, heparin).
Human IL-12 + IL-23 p40 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Human IL-12 + IL-23 p40 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human IL-12 + IL-23 p40 captured on the plate.
- アプリケーション適用あり: Sandwich ELISAmore details
- 試験プラットフォームMicroplate
製品の特性
- 保存方法Store at +4°C. Please refer to protocols.
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内容 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-Human IL12 + IL23 p40 monoclonal antibody 1 x 70µl Human IL-12 + IL-23 p40 Standard Lyophilized (4 ng/mL on reconstitution) 2 vials Microplate coated with anti-Human IL-12 + IL-23 p40 polyclonal antibodies (12 x 8 wells) 1 unit Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml - 研究分野
- 関連性Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
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別名
- CLMF
- CLMF p40
- CLMF2
see all -
参照データベース
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- SwissProt: P29460 Human
アプリケーション
Our Abpromise guarantee covers the use of ab119534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Human IL-12 + IL-23 p40 ELISA Kit 画像
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Typical Standard CurveRepresentative Standard Curve using ab119534.
プロトコール
Human IL-12 + IL-23 p40 ELISA Kit (ab119534) 使用論文
ab119534 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"