サンプル | N | 平均値 | SD | CV% |
---|---|---|---|---|
Overall | 8 | 4.7% |
サンプル | N | 平均値 | SD | CV% |
---|---|---|---|---|
Overall | 8 | 6.4% |
71
%
サンプルの種類 | 平均 % | 測定範囲 |
---|---|---|
Serum | 71 | 65% - 75% |
Abcam’s IL-12 + IL-23 p40 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-12 + IL-23 p40 concentrations in Cell culture supernatant, serum and plasma (citrate, heparin).
Human IL-12 + IL-23 p40 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Human IL-12 + IL-23 p40 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human IL-12 + IL-23 p40 captured on the plate.
内容 | 1 x 96 tests |
---|---|
20X Assay Buffer Concentrate | 1 x 5ml |
20X Wash Buffer Concentrate | 1 x 50ml |
Adhesive Films | 4 units |
Biotin-Conjugate anti-Human IL12 + IL23 p40 monoclonal antibody | 1 x 70µl |
Human IL-12 + IL-23 p40 Standard Lyophilized (4 ng/mL on reconstitution) | 2 vials |
Microplate coated with anti-Human IL-12 + IL-23 p40 polyclonal antibodies (12 x 8 wells) | 1 unit |
Sample Diluent | 1 x 12ml |
Stop Solution (1M Phosphoric acid) | 1 x 15ml |
Streptavidin-HRP | 1 x 150µl |
TMB Substrate Solution | 1 x 15ml |
Our Abpromise guarantee covers the use of ab119534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
ab119534 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"