Human IL-10 RB ELISA Kit (Interleukin-10 Receptor beta) (ab100550)


  • 製品名
    Human IL-10 RB ELISA Kit (Interleukin-10 Receptor beta)
  • 検出方法
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    4.12 pg/ml - 3000 pg/ml
  • 添加回収試験

    > 100 %

    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 129.1 120% - 140%
    Serum 121.9 110% - 129%
    Plasma 104.5 92% - 112%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-10 RB (Interleukin 10 Receptor beta) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IL-10 RB in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-10 RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム


  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human IL-10 RB 2 vials
    IL-10 RB Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-10 RB Standard 2 x 1ml
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野
  • 機能
    Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IL29.
  • 関連疾患
    Defects in IL10RB are the cause of inflammatory bowel disease type 25 (IBD25) [MIM:612567]. It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
  • 配列類似性
    Belongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
  • 細胞内局在
  • Information by UniProt
  • 別名
    • CDw210b
    • CRF2 4
    • CRF2-4
    • CRFB4
    • Cytokine receptor class II member 4
    • Cytokine receptor class-II member 4
    • Cytokine receptor family 2 member 4
    • D21S58
    • D21S66
    • I10R2_HUMAN
    • IL-10 receptor subunit beta
    • IL-10R subunit 2
    • IL-10R subunit beta
    • IL-10R2
    • IL-10RB
    • IL10 receptor subunit beta
    • IL10R subunit 2
    • IL10R subunit beta
    • IL10R2
    • IL10RB
    • Interleukin 10 receptor subunit 2
    • Interleukin 10 receptor subunit beta
    • Interleukin-10 receptor subunit 2
    • Interleukin-10 receptor subunit beta
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab100550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100550

  • Representative standard curve using ab100550



ab100550 has not yet been referenced specifically in any publications.

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