製品の概要

  • 製品名Human IL-10 ELISPOT Kit (without plates)
    IL10 キット 製品一覧
  • テスト
    5 x 96 well plate
  • サンプルの種類
    Suspension cells
  • アッセイタイプSandwich (qualitative)
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-10 Human ELISPOT kitis an in vitro ELISPOT assay designed for the qualitative measurement of IL-10 production and secretion in a single cell suspension.

     

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. ELISPOT assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

     

    The ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

     

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

     

    Recognizes natural human IL10

  • アプリケーション適用あり: ELISpotmore details
  • 試験プラットフォームReagents

製品の特性

  • 機能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 組織特異性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 配列類似性Belongs to the IL-10 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • CSIF
    • Cytokine synthesis inhibitory factor
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • TGIF
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISpot Use at an assay dependent dilution.

Human IL-10 ELISPOT Kit (without plates) (ab46549) 使用論文

ab46549 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab46549.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"