Human IL-10 ELISA Kit (Interleukin-10) High Sensitivity (ab46059)


  • 製品名Human IL-10 ELISA Kit (Interleukin-10) High Sensitivity
    IL10 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    サンプル N 平均値 SD CV%
    Overall 3 7.8%
    サンプル N 平均値 SD CV%
    Overall 3 10.2%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1.3 pg/ml
  • 検出範囲
    1.56 pg/ml - 50 pg/ml
  • 添加回収試験

    87 %

    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94 % - %
    Serum 80 59% - 93%

  • 全工程の試験時間
    3h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-10 (Interleukin-10) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in Human sera, plasmas, buffered solutions and cell culture media.

    A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-10.


  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate



  • 機能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 組織特異性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 配列類似性Belongs to the IL-10 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • TGIF
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab46059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-10 ELISA Kit (Interleukin-10) High Sensitivity 画像

  • Representative Standard Curve using ab46059


Human IL-10 ELISA Kit (Interleukin-10) High Sensitivity (ab46059) 使用論文

This product has been referenced in:
  • Hasegawa T  et al. Chafuroside B, an Oolong Tea Polyphenol, Ameliorates UVB-Induced DNA Damage and Generation of Photo-immunosuppression Related Mediators in Human Keratinocytes. PLoS One 8:e77308 (2013). Sandwich ELISA ; Human . Read more (PubMed: 24116222) »

See 1 Publication for this product

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Thank you for contacting us.

I am sorry about the continued problems that you have been experiencing using this kit. Unfortunately I do not have any further suggestions to help with this issue. I have created a credit note/refund for the o...

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I would like to suggest that you try using another kit to determine whether this is a product specific issue. If you provide me with the orginal Abcam order confirmation code I can send you a free of charge replacement for this product.

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I've passed this information on to the lab for you.

They feel that the data enclosed are pretty good, except the blank which is indeed a little bit high, and which should rather be considered as a zero. They also also would like to confirm w...

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Thank you for that additional information. The matrix effect could be called quenching. The high protein concentration of serum samples or components contained in samples can affect assay response to the analyte (IL-10) and give background. If yo...

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Thank you for contacting Abcam.

I have had the opportunity to speak with the lab regarding this issue. The low signal obtained may be linked to the use of human serum as standard curve diluent. Have you diluted the standards in human serum o...

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Thank you for your call today, and for letting us know about the trouble with this kit. As we discussed, please forward any data you have using this kit, and I will see if there are any suggestions that we can make to improve the results. I look forwar...

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Thank you for your call yesterday and for your patience while i have been in touch with the lab regarding these IL10 kits. Here is the reply that I received from my contact at the lab: "To be on the safe side regarding sensitivity, my opinion would be ...

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