Human IL-10 ELISA Kit (Interleukin-10) High Sensitivity (ab46059)

製品の概要

  • 製品名
    Human IL-10 ELISA Kit (Interleukin-10) High Sensitivity
    IL10 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3 7.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 10.2%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 1.3 pg/ml
  • 検出範囲
    1.56 pg/ml - 50 pg/ml
  • 添加回収試験

    87 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94 % - %
    Serum 80 59% - 93%

  • 全工程の試験時間
    3h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-10 (Interleukin-10) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in Human sera, plasmas, buffered solutions and cell culture media.


    A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-10.


    Get results in 90 minutes with Human IL-10 ELISA Kit (Interleukin-10) (ab185986) from our SimpleStep ELISA® range.


     


     


     

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab46059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab46059

プロトコール

参考文献

This product has been referenced in:
  • Sammour I  et al. The Effect of Gender on Mesenchymal Stem Cell (MSC) Efficacy in Neonatal Hyperoxia-Induced Lung Injury. PLoS One 11:e0164269 (2016). Read more (PubMed: 27711256) »
  • Han K  et al. Efficacy of double-coated probiotics for irritable bowel syndrome: a randomized double-blind controlled trial. J Gastroenterol N/A:N/A (2016). Read more (PubMed: 27178566) »

See all 3 Publications for this product

レビューと Q&A

Thank you for contacting us.

I am sorry about the continued problems that you have been experiencing using this kit. Unfortunately I do not have any further suggestions to help with this issue. I have created a credit note/refund for the o...

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I would like to suggest that you try using another kit to determine whether this is a product specific issue. If you provide me with the orginal Abcam order confirmation code I can send you a free of charge replacement for this product.

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I've passed this information on to the lab for you.

They feel that the data enclosed are pretty good, except the blank which is indeed a little bit high, and which should rather be considered as a zero. They also also would like to confirm w...

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Thank you for that additional information. The matrix effect could be called quenching. The high protein concentration of serum samples or components contained in samples can affect assay response to the analyte (IL-10) and give background. If yo...

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Thank you for contacting Abcam.

I have had the opportunity to speak with the lab regarding this issue. The low signal obtained may be linked to the use of human serum as standard curve diluent. Have you diluted the standards in human serum o...

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Thank you for your call today, and for letting us know about the trouble with this kit. As we discussed, please forward any data you have using this kit, and I will see if there are any suggestions that we can make to improve the results. I look forwar...

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Thank you for your call yesterday and for your patience while i have been in touch with the lab regarding these IL10 kits. Here is the reply that I received from my contact at the lab: "To be on the safe side regarding sensitivity, my opinion would be ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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