Human IL-10 ELISA Kit (Interleukin-10) (ab46034)

製品の概要

  • 製品名
    Human IL-10 ELISA Kit (Interleukin-10)
    IL10 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    sample 6 3.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    sample 6 7.3%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    12.5 pg/ml - 400 pg/ml
  • 添加回収試験

    111 %

  • 全工程の試験時間
    2h 40m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-10 (Interleukin-10) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in supernatants, buffered soloutions, serum and plasma samples.


    A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples. 


    This kit will recognize both endogenous and recombinant Human IL-10.


    Get results in 90 minutes with Human IL-10 ELISA Kit (Interleukin-10) (ab185986) from our SimpleStep ELISA® range.


     

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated anti IL-10 Red 1 x 400µl 2 x 400µl
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-10 Microplate (12 x 8 well strips) 1 unit 2 units
    IL-10 Standard (Lyophilized) Yellow 2 vials 4 vials
    Plastic Plate Covers 1 x 2 units 2 x 2 units
    Standard Diluent (Human Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 機能
    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 組織特異性
    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 配列類似性
    Belongs to the IL-10 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • TGIF
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab46034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • IL-10 detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297), with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Results shown after background signal was subtracted (duplicates +/- SD).
  • Representative standard curve using ab46034

プロトコール

参考文献

ab46034 has not yet been referenced specifically in any publications.

レビューと Q&A

IL10 human macrophage secretion on day 3

Excellent Excellent 5/5 (Ease of Use)
Abreviews
IL10 secretion by human macrophages isolated by negative selection in culture for 3 days.
Kit easy to use, the assay takes about 5.30hr, excellent specificity as shown by higher secretion by M2a macrophages (MSCF+IL4) and M2c (MCSF+TGFb) compared to the others subtypes (M0=MCSF M1=MSCF+IFNg DEX=MCSF+Dexamethason)
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投稿 Mar 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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