製品の概要

  • 製品名Human IL-1 R4 ELISA Kit (ST2)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    1.65 pg/ml - 1200 pg/ml
  • 添加回収試験

    97 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 98.43 91% - 105%
    Serum 109.6 98% - 117%
    Plasma 84.06 67% - 102%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IL-1 R4 (ST2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 R4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-1 R4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 R4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 R4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 R4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性ST2 is a member of a superfamily containing the Interleukin 1 (IL1) receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL1 receptor and Toll-like receptor 4 (TLR4) signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP.
  • 別名
    • DER4
    • FIT 1
    • Growth stimulation expressed
    • Homolog of mouse growth stimulation expressed
    • IL-1 R4
    • IL1R4
    • IL33R
    • Interleukin 1 receptor like 1
    • Interleukin 1 receptor related protein
    • Protein ST2
    • ST2S
    • T1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-1 R4 ELISA Kit (ST2) 画像

  • Representative standard curve using ab100563

  • Representative standard curve using ab100563

プロトコール

Human IL-1 R4 ELISA Kit (ST2) (ab100563) 使用論文

ab100563 has not yet been referenced specifically in any publications.

Product Wall

The detection range for ab100563 Human IL-1 R4 ELISA Kit (ST2) is based on the standard curve range and from testing of normal human serum with the kit.  Levels of IL-1 R4 in these samples using this kit were about 150 pg/ml).

Each kit manufac...

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The kit ab100563 recognizes the extracellular domain on the protein, aa 19-328.



J’ai contacté le laboratoire concernant le kit ab100563. L'immunogène utilisé pour créer les anticorps est une protéine recombinant humain IL-1R4 qui correspond à la région extracellulair...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"