製品の概要

  • 製品名
    Human IL-1 beta ELISA Kit (Interleukin-1 beta)
    IL1 beta キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    6 4.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    6 8.7%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    6.5 pg/ml
  • 検出範囲
    15.6 pg/ml - 500 pg/ml
  • 添加回収試験

    102.2 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 102.2 15.6pg/ml - 500pg/ml

  • 全工程の試験時間
    3h 45m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human IL-1 beta ab46052 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for IL-1 beta has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-1 beta concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-1 beta are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-1 beta present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-1 beta.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 2 x 96 tests 1 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 2 x 10ml 1 x 10ml
    Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml
    Biotinylated anti-IL-1 beta Red 2 x 400µl 1 x 400µl
    Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml
    Control Silver 4 vials 2 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-1 beta Microplate (12 x 8 well strips) 2 units 1 unit
    IL1 beta standard Yellow 4 vials 2 vials
    Standard Diluent (Serum) 2 x 7ml 1 x 7ml
    Stop Reagent Black 2 x 11ml 1 x 11ml
    Streptavidin-HRP 4 x 5µl 2 x 5µl
  • 研究分野
  • 機能
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 配列類似性
    Belongs to the IL-1 family.
  • ドメイン
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 細胞内局在
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 別名
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Standard curve of human IL-1β in standard diluent with background signal subtracted (duplicates; +/- SD).

  • IL-1β detected in supernatants from control THP-1 cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) and 1 ug x mL-1 LPS (Sigma) for the last 6 hours (P+L).

  • Representative Standard Curve using ab46052

プロトコール

参考文献

This product has been referenced in:
  • Sajid F & Bano S Pro inflammatory interleukins and thyroid function in Naswar (dipping tobacco) users: a case control study. BMC Endocr Disord 16:47 (2016). Sandwich ELISA ; Human . Read more (PubMed: 27515932) »

See 1 Publication for this product

レビューと Q&A

Thank you for your reply.

If you diluted the controls in the recommended volume of diluent, then you should have ended up with the concentration that is indicated on the control vials. Was there no concentration listed on the vials? If there ...

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Thank you for contacting Abcam.

For all three of those ELISA kits, there are standards and controls (4 vials of control per kit)provided and you should reconstitute those samples in the correct diluent depending on your sample types.
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Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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