製品の概要

  • 製品名Human IL-1 beta ELISA Kit (Interleukin-1 beta)
    IL1 beta キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.3 pg/ml
  • 検出範囲
    0.48 pg/ml - 100 pg/ml
  • 添加回収試験

    99 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 100.43 89% - 110%
    Serum 98.87 89% - 108%
    Plasma 99.66 90% - 107%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human IL-1 beta (Interleukin-1 beta) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 beta in serum, plasma, and cell culture supernatants. (Human IL-1 beta concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 配列類似性Belongs to the IL-1 family.
  • ドメインThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 細胞内局在Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 別名
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-1 beta ELISA Kit (Interleukin-1 beta) 画像

  • Hu IL-1 beta measured in biological fluids showing quantity (pg) per mL of tested sample (U937: stimulated 24h with 10ng/mL PMA and 6h with LPS 1microgram/mL -- THP-1: stimulated 24h with 10ng/mL PMA)

  • Representative standard curve using ab100562

  • Representative standard curve using ab100562

プロトコール

Human IL-1 beta ELISA Kit (Interleukin-1 beta) (ab100562) 使用論文

This product has been referenced in:
  • Al Batran R  et al. Evaluation of the effect of andrographolide on atherosclerotic rabbits induced by Porphyromonas gingivalis. Biomed Res Int 2014:724718 (2014). ELISA . Read more (PubMed: 25215291) »

See 1 Publication for this product

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Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

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Thank you for contacting us.

You will need 100ul of antibody for each well in 96 well plate. Say you are going to use all 96 wells so you will need final volume of antibody 9600ul (9.6 ml).

There is always a loss of few micro litres ...

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Which buffer you have to use depends on what kind of sample you are using. What kind of sample are you using?

Assay Diluent B should be used with the antibodies as Assay Diluent A contains 0.09% sodium azide as
preservative.This may inte...

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Yes, It is all free however they need to check if the items were paid in original order.

It is just a normal check so there is no need to worry, you will be getting a order confirmation soon.

Our accountsdepartment is holding the order at the moment. The order will be shipped once they authorize it, after checking thepayment etc. Please email mailto:creditcontrol@abcam.com for any order update.

Thanks!

Thank you for contacting us.

To prevent the shortageissue in the future, please advise your customer along with other customers to check all reagents before beginning the experiment and that if there is a volume shortage or any other issue to ...

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Thank you for your cooperation.

I have sent a request to lab for new kits and hoping to get an answer later this evening. I will confirm you the dispatch date.

I hope this info will be helpful.

Thank you for your email.

Regarding ab100562 have you used both kits? If not, then I will send you one replacement kit and one extra vial of HRP-standard. Could you let me know if you still have to use one kit of ab100562?

There are m...

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That's fine!

I will be sending you a replacement kitofab100562.

Could you check the HRP-streptavidin amount in ab100529.Is it OK?

Thank you for your email. I apologize for any inconvenience caused.

The volume 200ul is actually more than what is need for the assay; this extra volume is added to avoid any loss due to evaporation, insufficient centrifuging, etc. Actually on...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"