Human IL-1 alpha ELISA Kit (Interleukin-1 alpha) (ab100561)


  • 製品名Human IL-1 alpha ELISA Kit (Interleukin-1 alpha)
    IL1 alpha キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture extracts, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    0.82 pg/ml - 600 pg/ml
  • 添加回収試験

    95 %

    サンプルの種類 平均 % 測定範囲
    Cell culture extracts 94.78 96% - 106%
    Tissue Extracts 95.56 85% - 105%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human IL-1 alpha (Interleukin-1 alpha) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 alpha in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of
    IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate


  • 関連性IL1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
  • 細胞内局在Secreted
  • 別名
    • Hematopoietin 1
    • IL 1 alpha
    • Il 1a
    • IL1
    • IL1 ALPHA
    • IL1A
    • IL1F1
    • Ilia
    • Interleukin 1 alpha
    • Interleukin 1 alpha precursor
    • Interleukin1 alpha
    • OTTHUMP00000203692
    • OTTMUSP00000016430
    • Preinterleukin 1
    • Pro interleukin 1
    • Pro interleukin 1 alpha
    • Prointerleukin 1
    • RP23-160G19.8
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab100561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IL-1 alpha ELISA Kit (Interleukin-1 alpha) 画像

  • Representative standard curve using ab100561


Human IL-1 alpha ELISA Kit (Interleukin-1 alpha) (ab100561) 使用論文

ab100561 has not yet been referenced specifically in any publications.

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