Anti-Human IgM Fc fragment 抗体 [CH2] (ab772)

製品の概要

  • 製品名Anti-Human IgM Fc fragment antibody [CH2]
    Human IgM Fc fragment 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CH2] to Human IgM Fc fragment
  • 特異性This antibody reacts with Fc fragment of human IgM. Specificity was confirmed by Western blotting analysis of purified human IgM, under reducing conditions, and also by immunofluorescence staining of Daudi cells
  • アプリケーション適用あり: ICC/IF, Flow Cyt, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) (Human).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites by DEAE chromatography and precipitation methods.
  • ポリ/モノモノクローナル
  • クローン名CH2
  • ミエローマunknown
  • アイソタイプIgG1
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Flow Cyt
WB
ELISA
  • 追加情報ELISA: Use at an assay dependant concentration.
    Flow Cyt: Use at an assay dependant concentration.
    IF: Use at an assay dependant concentration.
    WB: Use at an assay dependant concentration.
    This antibody blocks binding of standard antibody to Fc fragment of human IgM and does not block binding of standard antibody to Fab fragment of human IgM.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
    • 細胞内局在Cell Membrane and Secreted
    • 参照データベース
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    Anti-Human IgM Fc fragment antibody [CH2] (ab772) 使用論文

    ab772 has not yet been referenced specifically in any publications.

    Product Wall

    The originator of these two antibodies has informed me that the anti Fab fragment antibody is better (IgG receptors bind IgG via Fc so it may be occupied). The Fc fragment is not good for IgM bound to the B-cell surface, but is great for soluble IgM.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"