製品の概要

  • 製品名Anti-Human IgG antibody (FITC)
    Human IgG 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Human IgG (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性Low background fluorescence of normal tissue in contrast to cases of autoimmune disease, deposited immunoglobulin IgG. No cross reactivity with albumin fractions of human serum. No cross reactivity with albumin fractions of animal species.
  • アプリケーション適用あり: IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human IgG.

  • 特記事項

    The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel filtation and ion exchange chromatography to clear unbound conjugate. F/P ratio: 4-6 mol Fluorescein per 1 mol of goat IgG.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, 0.1M Sodium chloride, 0.01M Sodium phosphate, pH 7.5
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorbed by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/40.
Flow Cyt Use at an assay dependent concentration. ab37394-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • 細胞内局在Secreted
  • 参照データベース
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Anti-Human IgG antibody (FITC) (ab81051) 使用論文

This product has been referenced in:
  • Gramlich OW  et al. Enhanced insight into the autoimmune component of glaucoma: IgG autoantibody accumulation and pro-inflammatory conditions in human glaucomatous retina. PLoS One 8:e57557 (2013). ICC/IF ; Human . Read more (PubMed: 23451242) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"