製品の概要

  • 製品名Human IGFBP4 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.25 ng/ml
  • 検出範囲
    0.41 ng/ml - 100 ng/ml
  • 添加回収試験

    95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 97.38 86% - 106%
    Serum 93.98 83% - 105%
    Plasma 94.69 94% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IGFBP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP4 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IGFBP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 配列類似性Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • AI875747
    • BP 4
    • BP4
    • Deb2
    • HT29 IGFBP
    • IBP 4
    • IBP-4
    • IBP4
    • IBP4_HUMAN
    • IGF binding protein 4
    • IGF BP4
    • IGF-binding protein 4
    • IGFBP 4
    • IGFBP-4
    • IGFBP4
    • Insulin like growth factor binding protein 4
    • Insulin-like growth factor-binding protein 4
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IGFBP4 ELISA Kit 画像

  • Representative standard curve using ab100542

  • Representative standard curve using ab100542

プロトコール

Human IGFBP4 ELISA Kit (ab100542) 使用論文

ab100542 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your response.

I would suggest reading through carefully Section 2 (Assay Summary) and Section 6 (Preparation of the Reagents).

I hope this will be useful for you.

Thank you for your enquiry.

There is a Protocol booklet which can be found under the Protocol section (at the bottom of the datasheet - blue hyperlink) OR under the Protocol Tab (top right side) which can be downloaded. Could you please advis...

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Thank you for contacting us.

We have in our catalogue the IGFBP4 protein (active form). The reference is ab125753 and the link to the datasheet is http://www.abcam.com/ab125753.

A tissue that is recommended as a positive control for ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"