製品の概要

  • 製品名Human IGFBP2 ELISA Kit
    IGFBP2 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    97 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 100.25 86% - 105%
    Serum 95.77 84% - 103%
    Plasma 97.59 85% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IGFBP2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 配列類似性Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • ドメインThe C-terminus is required for IGF-binding and growth inhibition.
  • 翻訳後修飾O-glycosylated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • BP 2
    • BP2
    • IBP 2
    • IBP-2
    • IBP2
    • IBP2_HUMAN
    • IGF binding protein 2
    • IGF BP53
    • IGF-binding protein 2
    • IGF-BP53
    • IGFBP-2
    • IGFBP2
    • IGFBP53
    • Insulin Like Growth Factor Binding Protein 2
    • Insulin like growth factor binding protein 2 36kDa
    • Insulin like growth factor-binding protein 2 precursor
    • Insulin-like growth factor-binding protein 2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human IGFBP2 ELISA Kit 画像

  • Representative standard curve using ab100540

  • Representative standard curve using ab100540

プロトコール

Human IGFBP2 ELISA Kit (ab100540) 使用論文

This product has been referenced in:
  • Chang YC  et al. The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress. Front Aging Neurosci 6:191 (2014). Sandwich ELISA ; Human . Read more (PubMed: 25136316) »
  • Sunderic M  et al. Protein molecular forms of insulin-like growth factor binding protein-2 change with aging. Exp Gerontol 58C:154-158 (2014). Read more (PubMed: 25106097) »

See all 3 Publications for this product

Product Wall

Thank you and your customer for your inquiry.

Although we have not tested the Human ab100540 and ab100544ELISA kits with ascite fluid,these kits are very accommodating to many different samples types so the ascites will most likely work.
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Thank you for contacting Abcam.

For both of these ELISA kits we do recommend that you make the wash buffer up fresh before every use and we haven't tested the long term stability of the buffer when diluted to 1X. It should be good if stored o...

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Thank you for contacting us.

Because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products. Therefore, I am afraid I cannot send you a free sample of the IGFBP2 Human ELISA Kit ab100540.
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I apologize for the delay in getting back to you in regards to this issue, and I appreciate your patience as I have been discussing this situation with my colleagues. I can understand your frustration and disappointment as the results have been unexpec...

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Thank you very much for the data. I am working with some of my colleagues to resolve this as quickly as possible, and I appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Thank you for your reply. I have found the order with the additional kits that you mentioned. In order to process this refund, I will need a little bit more technical information. Have you tried all of these kits, or did you test 1 from each set and th...

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Thank you for your reply, and I apologize that these kits did not work well for you. I did not find the attached summary of the test results, could you please send these again? We do fully guarantee these kits to work, so I have issued credit note *** ...

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Thank you for your patient. I just had a response from my lab colleagues. They confirmed that all three kits were designed against the mature form of the protein (which is the full amino acid sequence minus the signalling peptide). As the epito...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"