Human IGFBP2 ELISA Kit (ab100540)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human IGFBP2 ELISA Kit
IGFBP2 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 20 pg/ml -
検出範囲
8.23 pg/ml - 6000 pg/ml -
添加回収試験
97 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 100.25 86% - 105% Serum 95.77 84% - 103% Plasma 97.59 85% - 104% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IGFBP2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
特記事項
Optimization may be required with urine samples
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IGFBP2 2 vials IGFBP2 Microplate (12 x 8 wells) 1 unit Recombinant Human IGFBP2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
機能
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
ドメイン
The C-terminus is required for IGF-binding and growth inhibition. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- BP 2
- BP2
- IBP 2
see all -
参照データベース
- Entrez Gene: 3485 Human
- Omim: 146731 Human
- SwissProt: P18065 Human
- Unigene: 438102 Human
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab100540 は 4 報の論文で使用されています。
- Al-Regaiey K et al. Effects of gastric sleeve surgery on the serum levels of GH, IGF-1 and IGF-binding protein 2 in healthy obese patients. BMC Gastroenterol 20:199 (2020). PubMed: 32586279
- Chang YC et al. The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress. Front Aging Neurosci 6:191 (2014). Sandwich ELISA ; Human . PubMed: 25136316
- Sunderic M et al. Protein molecular forms of insulin-like growth factor binding protein-2 change with aging. Exp Gerontol 58C:154-158 (2014). PubMed: 25106097
- Nedic O et al. Detection and identification of oxidized insulin-like growth factor-binding proteins and receptors in patients with colorectal carcinoma. Free Radic Biol Med 65:1195-200 (2013). Sandwich ELISA ; Human . PubMed: 24051179