製品の概要

  • 製品名
    Human IGF1 ELISA Kit
    IGF1 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 12%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 0.2 ng/ml
  • 検出範囲
    0.1 ng/ml - 30 ng/ml
  • 添加回収試験

    97 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 102.5 98% - 110%
    Serum 119.6 105% - 138%
    Plasma 105.4 81% - 121%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s IGF1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IGF1 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IGF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


     Get results in 90 minutes with Human IGF1 ELISA Kit (ab211651) from our SimpleStep ELISA® range.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab100545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • Representative standard curve using ab100545.

  • IGF1 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-64 fold.

  • Human IGF1 standard curve: mean of duplicates (+/- SD)

プロトコール

参考文献

This product has been referenced in:
  • Baskerville CL  et al. Comparison and validation of ELISA assays for plasma insulin-like growth factor-1 in the horse. Open Vet J 7:75-80 (2017). Read more (PubMed: 28540255) »
  • Groër MW  et al. Allostatic Perspectives in Women Veterans With a History of Childhood Sexual Assault. Biol Res Nurs 18:454-64 (2016). Read more (PubMed: 27067613) »

See all 2 Publications for this product

レビューと Q&A

ab100545-Human IGF1 ELISA Kit for Human Saliva

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This kit worked successfully to quantify IGF1 protein in human saliva. We obtained valid results for all of our wells and were able to use these results to compare to them to amounts of BDNF and cortisol in saliva. The results from this kit greatly contributed to my senior thesis project, which looked at differences in IGF1 levels across a 3-month exercise intervention in older adults. We will very likely be ordering this kit again in for future analyses of IGF1.
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Abcam user community

Verified customer

投稿 Apr 14 2017

This kit is designed to measure free IGF1. IGF-1 is usually bound to IGFBPs in the serum/plasma, so if you would like to measure total IGF1, it is advisable to release the analytes by performing an extraction procedure such as acid-ethanol treatment (P...

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Unfortunately the immunogen sequence for the antibodies used in the kit ab100545 is not available. Nor do we have cross reactivity data for kangaroo species. If it helps, the capture antibody used is monoclonal and the detection antibody is polyclonal....

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L’anticorps de capture de ce kit ne reconnait pas l’IGF-1 du rat. Pour l’anticorps de détection, la réactivé avec la protéine du rat n’a pas été testé donc malheureusement, ce kit...

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The host species for the capture antibody is mouse and the detection antibody is goat.

Vielen Dank für Ihre Antwort und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit diesem Kit hatten. Da die Standardkurve gutgelungen ist, wird höchswahr...

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Vielen Dank für Ihren Anruf.

Leider ist es so, wie ich befürchtet habe, wir können leider keine Angabe zu diesen Kits mit HepG2 Zellen, bzw mit anderen Leberzelllinien machen. Grundsätzlich spricht aber nichts gegen die Ver...

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Thank you for your enquiry.

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I can confirm this kit recognizes the mature form of VEGF-C (aa 103-227). Based on sequence similarity, the kit is also expected to detect the pro-form but this has not ...

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Thank you for contacting us. I have been in contact with the lab regarding your inquiry.They are not familiar with the Immulite 1000. However, as long as the system can perform the protocol as written, we do not see why it would not work. As this sy...

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Thank you for your inquiry.

I have contacted the lab with your enquiry and here is their advise fot you:

"What type of sample is the customer testing? If they are testing serum or plasma, are they performing the recommended ext...

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1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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