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Our Abpromise guarantee covers the use of ab65866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.
IgE has been used in a variety of cell stimulation assays, including the following cell types:
This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.
1 mg of ab65866 is 380,000± 50,000 IU/ml.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 0.15M Sodium chloride, 0.01M PBS, pH 7.4
Background-subtracted data values (mean +/- SD) are graphed.
Lane 1: MW markers
Lane 2: ab65866
Lane 3: Native IgE purified from myeloma plasma, kappa
IgE proteins were diluted from stock to 5µg/well. An aliquot of each protein was loaded onto 4-12% gradient and gels were subsequently run for 65 mins at 120V.