Anti-Human Ig light chain 抗体 [7A9] (ab1942)


  • 製品名Anti-Human Ig light chain antibody [7A9]
    Human Ig light chain 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7A9] to Human Ig light chain
  • 特異性Human IgA, IgG and IgM.
  • アプリケーション適用あり: Conjugation, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Mixture of light chains of human immunoglobulin.

  • ポジティブ・コントロール
    • IHC-P: FFPE human spleen tissue sections.
  • 特記事項Concentration varies from lot to lot and can be provided on request.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purity tested by electrophoresis.
  • ポリ/モノモノクローナル
  • クローン名7A9
  • ミエローマSp2/0
  • アイソタイプIgG2a
  • 研究分野


Our Abpromise guarantee covers the use of ab1942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
  • 追加情報Conjugation: Use at an assay dependent dilution.

    Specific antibody titer is 1/1000000 by indirect ELISA

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性There are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain. In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species. Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment.
    • 細胞内局在Cell Membrane: single-pass type I membrane protein and Secreted
    • 参照データベース
    • 別名
      • HCAK1 antibody
      • Ig kappa chain antibody
      • IGKC antibody
      • Immunoglobulin kappa constant antibody
      • immunoglobulin kappa light chain (VJ) antibody
      • kappa 1 immunoglobulin light chain antibody
      • KM antibody
      • MGC111575 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    Anti-Human Ig light chain antibody [7A9] 画像

    • IHC image of Human Ig light chain staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1942, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-Human Ig light chain antibody [7A9] (ab1942) 使用論文

    ab1942 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 7 µg
    Gel Running Conditions Non-reduced Denaturing (4-12% Bis Tris NuPage)
    Sample Human Recombinant protein (Monoclonal human IgG antibody produced in lab)
    Specification Monoclonal human IgG antibody produced in lab
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

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    投稿 Nov 07 2013