製品の概要

  • 製品名Human ICAM3 ELISA Kit (CD50)
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Sample A 8 19.75ng/ml 0.172 0.86%
    Sample B 8 2.83ng/ml 0.099 3.49%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Sample A 36 19.51ng/ml 0.391 1.99%
    Sample B 36 2.71ng/ml 0.176 6.27%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.3 ng/ml
  • 検出範囲
    0.62 ng/ml - 20 ng/ml
  • 全工程の試験時間
    1h 40m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s ICAM3 (CD50) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ICAM3 in serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for ICAM3 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM3 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM3 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM3 present in the samples. 

    This kit will recognize both endogenous and recombinant Human ICAM3.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 保存方法Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-ICAM-3 (CD50) Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    ICAM3 Control Silver 2 vials 4 vials
    ICAM3 Microplate (12 x 8 well strips) 1 unit 2 units
    ICAM3 Standard Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 関連性ICAM3 is a member of the intercellular adhesion molecule family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule.
  • 細胞内局在Cell Membrane; single-pass type I membrane protein
  • 別名
    • CD50
    • CDW50
    • ICAM 3
    • ICAM R
    • ICAMR
    • Intercellular adhesion molecule 3
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human ICAM3 ELISA Kit (CD50) 画像

  • Representative Standard Curve using ab46024

プロトコール

Human ICAM3 ELISA Kit (CD50) (ab46024) 使用論文

ab46024 has not yet been referenced specifically in any publications.

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The expiration date that we assigned for that lot is October 2013. However, our guarantee is in effect for only 6 months after the purchase of the kit, so I recommend contacting us immediately if you have any iss...

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Thank you for your patience.

The lab let me know that it is not necessary to use glass vials. Plastic vials suit perfectly. The crucial point is cleanness.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"