製品の概要

  • 製品名
    Human ICAM2 ELISA Kit (CD102)
    ICAM2 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 8 23.62U/ml 0.277 1.17%
    B 8 2.96U/ml 0.86 2.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 33 23.26U/ml 0.668 2.87%
    B 33 3.12 0.218 6.98%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.2 U/ml
  • 検出範囲
    0.75 U/ml - 24 U/ml
  • 全工程の試験時間
    1h 40m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human ICAM2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble Inter Cellular Adhesion Molecule -2 (ICAM2) in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for ICAM2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM2 present in the samples.

    This kit will recognize both endogenous and recombinant Human ICAM2.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 ラベル 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-ICAM2 Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    ICAM2 Microplate (12 x 8 well strips) 1 unit 2 units
    ICAM2 Standard (Lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究分野
  • 関連性
    ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • 細胞内局在
    Type 1 membrane protein
  • 別名
    • CD102
    • ICAM 2
    • Intercellular adhesion molecule 2
    • Ly60
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab46080

プロトコール

参考文献

ab46080 has not yet been referenced specifically in any publications.

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