製品の概要

  • 製品名Human ICAM2 ELISA Kit (CD102)
    ICAM2 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 8 23.62U/ml 0.277 1.17%
    B 8 2.96U/ml 0.86 2.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 33 23.26U/ml 0.668 2.87%
    B 33 3.12 0.218 6.98%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.2 U/ml
  • 検出範囲
    0.75 U/ml - 24 U/ml
  • 全工程の試験時間
    1h 40m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human ICAM2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble Inter Cellular Adhesion Molecule -2 (ICAM2) in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for ICAM2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM2 present in the samples.

    This kit will recognize both endogenous and recombinant Human ICAM2.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 関連性ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • 細胞内局在Type 1 membrane protein
  • 別名
    • CD102
    • ICAM 2
    • Intercellular adhesion molecule 2
    • Ly60
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human ICAM2 ELISA Kit (CD102) 画像

  • Representative Standard Curve using ab46080

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Human ICAM2 ELISA Kit (CD102) (ab46080) 使用論文

ab46080 has not yet been referenced specifically in any publications.

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