製品の概要

  • 製品名
    Human ICAM1 ELISA Set (without plates)
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 0.1 ng/ml
  • 検出範囲
    0.25 ng/ml - 8 ng/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s ICAM1 Human in vitro ELISA Set is designed for the quantitative measurement of ICAM1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA set is intended to measure human ICAM1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    The ICAM1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human ICAM1.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Reagents

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-ICAM1 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    ICAM1 Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
    ICAM1 Standard 5 vials 10 vials 15 vials
    Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
  • 研究分野
  • 機能
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • 配列類似性
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻訳後修飾
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 別名
    • BB2
    • CD54
    • ICAM-1
    • ICAM1
    • ICAM1_HUMAN
    • Intercellular adhesion molecule 1
    • Major group rhinovirus receptor
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab47349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA
  • 追加情報
    ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 画像

    参考文献

    ab47349 has not yet been referenced specifically in any publications.

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab47349.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録