Human I-309 ELISA Kit (CCL1) (ab100536)


  • 製品名Human I-309 ELISA Kit (CCL1)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 4 pg/ml
  • 検出範囲
    4.12 pg/ml - 3000 pg/ml
  • 添加回収試験

    95 %

    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 79.9 70% - 103%
    Serum 106.9 97% - 116%
    Plasma 97.65 88% - 110%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s I-309 (CCL-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human I-309 (CCL-1) in serum, plasma and cell culture supernatants. (Human I-309 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human I-309 coated on a 96-well plate. Standards and samples are pipetted into the wells and
    I-309 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human I-309 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-309 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate


  • 機能Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • BF534335
    • C C motif chemokine 1
    • C-C motif chemokine 1
    • Ccl1
    • CCL1_HUMAN
    • CCR8 ligand
    • Chemokine CC Motif Ligand 1
    • Inflammatory cytokine I-309
    • P500
    • RP23-446K18.2
    • SCYA1
    • SISe
    • Small inducible cytokine A1
    • Small-inducible cytokine A1
    • T lymphocyte secreted protein I-309
    • T lymphocyte-secreted protein I-309
    • TCA3
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab100536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human I-309 ELISA Kit (CCL1) 画像

  • Representative standard curve using ab100536


Human I-309 ELISA Kit (CCL1) (ab100536) 使用論文

ab100536 has not yet been referenced specifically in any publications.

Product Wall

CCL1-I309 human macrophages and DC secretion

Excellent Excellent 5/5 (Ease of Use)
CCL1-I309 secretion by human macrophages and DC isolated by negative selection in culture for 3 & 7 days.
Kit easy to use, the assay takes about 6hr.
good specificity as shown by CCL1 secretion in all macrophages subtypes (M0=MCSF M1=MCSF+IFNg M2a=MCSF+IL4 DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb) and DC (GMCSF+IL4) on day 3 & 7.

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投稿 Apr 09 2014

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I am sorry that this product did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to cont...

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Thank you for forwarding your customer's data. We would like to offer some tips to improve the results seen with the standard curve.For clarity, I have separated the evaluation of the data by the two experiments.

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