製品の概要

  • 製品名Human HVEM ELISA Kit (TNFRSF14 )
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 30 pg/ml
  • 検出範囲
    32.77 pg/ml - 8000 pg/ml
  • 添加回収試験

    88 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 85.34 69% - 98%
    Serum 90.17 81% - 97%
    Plasma 90.83 73% - 112%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s HVEM Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HVEM in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human HVEM coated on a 96- well plate. Standards and samples are pipetted into the wells and HVEM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HVEM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HVEM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
  • 組織特異性Widely expressed, with the highest expression in lung, spleen and thymus.
  • 配列類似性Contains 3 TNFR-Cys repeats.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • CD270
    • Herpes virus entry mediator A
    • Herpesvirus entry mediator A
    • HveA
    • TNFRSF14
    • TNR14_HUMAN
    • TR2
    • Tumor necrosis factor receptor superfamily member 14
    • Tumor necrosis factor receptor-like 2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human HVEM ELISA Kit (TNFRSF14 ) 画像

  • Representative Standard Curve using ab113335

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Human HVEM ELISA Kit (TNFRSF14 ) (ab113335) 使用論文

ab113335 has not yet been referenced specifically in any publications.

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