製品の概要

製品の詳細

  • 由来Synthetic
  • アミノ酸配列
    • アクセッション番号P62805
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab14963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 製品の状態Liquid
  • 備考

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

関連情報

  • 別名
    • dJ160A22.1
    • dJ160A22.2
    • dJ221C16.1
    • dJ221C16.9
    • FO108
    • H4
    • H4.k
    • H4/a
    • H4/b
    • H4/c
    • H4/d
    • H4/e
    • H4/g
    • H4/h
    • H4/I
    • H4/j
    • H4/k
    • H4/m
    • H4/n
    • H4/p
    • H4_HUMAN
    • H4F2
    • H4F2iii
    • H4F2iv
    • H4FA
    • H4FB
    • H4FC
    • H4FD
    • H4FE
    • H4FG
    • H4FH
    • H4FI
    • H4FJ
    • H4FK
    • H4FM
    • H4FN
    • H4M
    • HIST1H4A
    • HIST1H4B
    • HIST1H4C
    • HIST1H4D
    • HIST1H4E
    • HIST1H4F
    • HIST1H4H
    • HIST1H4I
    • HIST1H4J
    • HIST1H4K
    • HIST1H4L
    • HIST2H4
    • HIST2H4A
    • Hist4h4
    • Histone 1 H4a
    • Histone 1 H4b
    • Histone 1 H4c
    • Histone 1 H4d
    • Histone 1 H4e
    • Histone 1 H4f
    • Histone 1 H4h
    • Histone 1 H4i
    • Histone 1 H4j
    • Histone 1 H4k
    • Histone 1 H4l
    • Histone 2 H4a
    • histone 4 H4
    • Histone H4
    • MGC24116
    see all
  • 機能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性Belongs to the histone H4 family.
  • 翻訳後修飾Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt

Human Histone H4 peptide (ab14963) 使用論文

This product has been referenced in:
  • FitzGerald J  et al. Regulation of the DNA damage response and gene expression by the Dot1L histone methyltransferase and the 53Bp1 tumour suppressor. PLoS One 6:e14714 (2011). Blocking . Read more (PubMed: 21383990) »
  • Wang Y  et al. Regulation of Set9-mediated H4K20 methylation by a PWWP domain protein. Mol Cell 33:428-37 (2009). Read more (PubMed: 19250904) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam regarding our Histone H4 peptides. The specific sequences of these peptides are considered proprietary and are not available on our website. The peptides are short, 8aa peptides and do contain a C terminal cysteine. If ...

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Thank you for contacting Abcam. The immunogen sequence for ab14963 is **********************. Please let me know if there is anything else I can help you with.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"