The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
H1 0 histone
H1 histone family member 0
H1 histone family member 1
H1 histone family member 3
H1 histone family member 5
H1 histone family member N testis specific
H1 histone family member O oocyte specific
H1 histone family member X
Histone 1 H1a
Histone 1 H1b
Histone 1 H1c
Histone 1 H1d
Histone 1 H1e
Histone 1 H1t
Oocyte specific histone H1
Oocyte specific linker histone H1
Oocyte-specific histone H1
Oocyte-specific linker histone H1
Testicular H1 histone
May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
Belongs to the histone H1/H5 family. Contains 1 H15 (linker histone H1/H5 globular) domain.