製品の概要

  • 製品名
    Human HGF ELISA Kit
    HGF キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 3 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    > 98 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 98.35 89% - 108%
    Serum 108.28 96% - 120%
    Plasma 107.56 95% - 119%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s HGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HGF in serum, plasma (EDTA and citrate), and cell culture supernatants.

    This assay employs an antibody specific for Human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • HuHGF measured in biological fluids showing quantity (pg) per mL of tested sample.

  • Representative standard curve using ab100534

  • Representative standard curve using ab100534

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参考文献

This product has been referenced in:
  • Nishida S  et al. Prostate cancer stem-like cells/cancer-initiating cells have an autocrine system of hepatocyte growth factor. Cancer Sci 104:431-6 (2013). Human . Read more (PubMed: 23320511) »

See 1 Publication for this product

レビューと Q&A

HGF ELISA in cell culture media

Good Good 4/5 (Ease of Use)
Abreviews
HGF ELISA worked well with cell cultured media.
I used the the kit as instruction provided in kit and cultured cell line media was used as it is without diluting.
Username

Abcam user community

Verified customer

投稿 Apr 21 2017

Thank you for your inquiry. I have answered your questions below:

Does this kit detect total HGF - the single chain precursor of HGF (scHGF) and the activated heterodimer as well?

The exact form of HGF recognized has not been tested, ...

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I am sorry for the delay in getting back to you. I now have further data to share with you from the HGF Human ELISA Kit (ab100534).

Please find attached an excel spreadsheet. In the first sheet there is the data collected from two subsequent a...

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I am sorry for the delay in getting back to you. I now have further information to share with you in relation the variation seem by your customer when using the HGF Human ELISA Kit (ab100534).

The source of this kti ahve had a look at the resu...

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Thank you for contacting us.

The reconstituted standard is stable for ˜1 week if stored frozen (-20°C - -80°C). Please ensure avoiding multiple freeze – thaw cycles. We do provide 2 vials of the standard recombinant protein to m...

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Thank you for confirming that. xxxx is currently not in the office but I can help with this case.

I have now added the replacement kit to the order number xxxxxx. Please be aware that the kit may be moved from this order before shipping to be...

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Thank you for your reply.

It can't be that the buffer has the same consistency as glycerol!As we do not have separatecomponents, I would like to offer your customer a FOCR instead.

Please let me know if this is acceptable.

Thank you for sending in the data. The standard curve does still contain strong linearity when including the higher concentrated standards, 222.2 pg/ml and 666.7 pg/ml. The highest standard (2000 pg/ml) did give a reading that affects the linearity dow...

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Thank you for contacting us.

260 uL of Assay Diluent A or 1x Assay Diluent B should be added to all of the vials except the first one. The first vial should contain 390 uL of the Standard that was prepared in 600 uL of Assay Diluent A or B. T...

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It is difficult to recommend a specific speed for a "gentle shaking". The plate should just bemoving to get a homogenous solution and not too fast to avoid a spillage.I hope this helps.

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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