製品の概要

製品の詳細

  • 由来Synthetic
  • アミノ酸配列
    • アクセッション番号P81172
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab31875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking - Blocking peptide for Anti-Hepcidin-25 antibody (ab30760), Anti-Hepcidin-25 antibody (ab75883)

    ELISA

  • 精製度70 - 90% by HPLC.

  • 製品の状態Liquid
  • 備考

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

関連情報

  • 別名
    • Hamp
    • HEPC
    • HEPC_HUMAN
    • Hepc20
    • Hepc25
    • HEPCIDIN
    • Hepcidin 20
    • Hepcidin 25
    • Hepcidin antimicrobial peptide
    • Hepcidin-20
    • Hepcidin25
    • HFE2
    • HFE2B
    • LEAP 1
    • LEAP-1
    • LEAP1
    • Liver expressed antimicrobial peptide
    • Liver-expressed antimicrobial peptide 1
    • PLTR
    • Putative liver tumor regressor
    see all
  • 機能Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
    Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
  • 組織特異性Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
  • 関連疾患Hemochromatosis 2B
  • 配列類似性Belongs to the hepcidin family.
  • 細胞内局在Secreted.
  • Information by UniProt

Human Hepcidin-25 peptide (ab31875) 使用論文

This product has been referenced in:
  • Xiong XY  et al. Toll-Like Receptor 4/MyD88-Mediated Signaling of Hepcidin Expression Causing Brain Iron Accumulation, Oxidative Injury, and Cognitive Impairment After Intracerebral Hemorrhage. Circulation 134:1025-1038 (2016). Read more (PubMed: 27576776) »
  • McCarthy RC & Kosman DJ Glial cell ceruloplasmin and hepcidin differentially regulate iron efflux from brain microvascular endothelial cells. PLoS One 9:e89003 (2014). Read more (PubMed: 24533165) »

See all 2 Publications for this product

Product Wall

Application ELISA
Review text: Used in conjunction with ab30760 at does dependent concentration as a positive control.
Sample: Mouse Recombinant protein (Mouse Primary neurons)
Specification: Mouse Primary neurons
Blocking step: BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Type: Direct

Other product details
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: ([2.93g Sodium Bicarbonate (NaHCO3), 1.5

Primary antibody (in addition to 'Hepcidin-25 peptide')
Dilution: 1/5000

Secondary antibody
Name: Non-Abcam antibody was used: Dako goat anti rabbit HRP antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/50000
Username

Abcam user community

Verified customer

投稿 Oct 09 2012

Thank you for contacting Abcam regarding ab31875.


The buffer contains the following:


30 mM HEPES
150 mM NaCl
2 mM EDTA
0.001% Tween 20
At pH 6.75


I hope this information is helpful. Please do no...

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Merci pour la confirmation.

Comme convenu, j'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN est *******.

Voici les mani&eg...

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Merci de nous avoir contactés.

Je suis désolé que le produit ne fonctionne pas.

L'explication est très probablement la suivante: la longueur de ce peptide n'est que de 25 acides aminés (aa 60-84 de l...

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Thank you for contacting us.

The peptide should already be in solution. Is that not the case? You should have 100ul. Be sure to centrifuge the tube to pulll down any solution trapped in the threads of the tube.

If you want to furt...

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Thank you for contacting us.

The product ab31875 is a synthetic 25 amino acid peptide that is not, to our knowledge, active. This peptide has been used to produce the antibody ab75883, which is validated for use on mouse and human samples in ...

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Thank you for your reply.

I am still concerned that the resolving conditions are not suitable for such a small protein. I will send on the peptide as an exception. However, I am not sure how it will be used for blocking if a band of the appr...

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Thanks you for your email. Dr. Tanya is away this week so I am looking after her inbox. The peptide sequence for ab31876 corresponds to amino acid XXXX and peptide sequence for ab31875 corresponds to XXXX amino acids. The antibody cross ...

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Thank you for your enquiry and your interest in our products. I have conducted a quick blast and the peptide sequence picks up both hepcidin and hepcidin 25. The peptide sequence corresponds to aas 60-84 of the full length protein. The antibod...

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Thank you for contacting us. This peptide was synthesised on solid phase, using Fmoc-protected amino acids. Amino acid side-chains are protected. The full length peptide is cleaved from the resin and fully deprotected using 95% TFA and scavenger mi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"