製品の概要

  • 製品名Human Hemopexin ELISA Kit (HPX)
    Hemopexin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.3%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 6 ng/ml
  • 検出範囲
    6.25 ng/ml - 400 ng/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    3h 30m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Hemopexin (HPX) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Hemopexin levels in Human cell culture supernatants, milk, saliva and urine.


    A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Hemopexin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Hemopexin captured in plate.


    For higher sensitivity in only 90 minutes, we recommend Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 配列類似性Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • 翻訳後修飾N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Beta 1B glycoprotein
    • Beta-1B-glycoprotein
    • FLJ56652
    • HEMO
    • HEMO_HUMAN
    • Hemopexin
    • Hpx
    • HX
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Hemopexin ELISA Kit (HPX) 画像

  • Hemopexin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human saliva diluted 60-120 fold. Human milk diluted 300-600 fold. Human urine diluted 10 fold - below detection. Cell supernatants tested neat.

  • Representative Standard Curve using ab108860.

プロトコール

Human Hemopexin ELISA Kit (HPX) (ab108860) 使用論文

This product has been referenced in:
  • Zager RA  et al. Renal cortical hemopexin accumulation in response to acute kidney injury. Am J Physiol Renal Physiol 303:F1460-72 (2012). Sandwich ELISA . Read more (PubMed: 22993068) »

See 1 Publication for this product

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