製品の概要

  • 製品名Human Hemopexin ELISA Kit (HPX)
    Hemopexin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.4%
  • サンプルの種類
    Milk, Serum, Plasma
  • アッセイタイプCompetitive
  • 検出感度
    > 0.7 µg/ml
  • 検出範囲
    0.078 µg/ml - 5 µg/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    2h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Hemopexin (HPX) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of hemopexin levels in plasma and serum.


    A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Hemopexin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Hemopexin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 配列類似性Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • 翻訳後修飾N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Beta 1B glycoprotein
    • Beta-1B-glycoprotein
    • FLJ56652
    • HEMO
    • HEMO_HUMAN
    • Hemopexin
    • Hpx
    • HX
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

Human Hemopexin ELISA Kit (HPX) 画像

  • Hemopexin measured in various biological fluids showing quantity (μg) per mL of tested sample

  • Representative Standard Curve using ab108859.

プロトコール

Human Hemopexin ELISA Kit (HPX) (ab108859) 使用論文

This product has been referenced in:
  • Evans C  et al. Impairment of neutrophil oxidative burst in children with sickle cell disease is associated with heme oxygenase-1. Haematologica N/A:N/A (2015). Read more (PubMed: 26315932) »
  • Insenser M  et al. Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: a nontargeted proteomic study. PLoS One 9:e100902 (2014). Read more (PubMed: 24979072) »

See all 2 Publications for this product

Product Wall

Hemopexin (HPX) Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Sample: EDTA human plasma stored at -80ºC
Amount of Sample: 3 l
Type of Kit Used: ELISA
Manufacturer instructions were followed.
Additional data
We diluted 3 l of plasma in 1200 l of 1XDiluent N (1:400)
Washings were done on a Microplate Washer machine with 300 l of wash buffer.
The standards data generated an excellent curve.
All samples assayed showed data inside the curve.
Username

Abcam user community

Verified customer

投稿 Apr 08 2015

Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2)...

Read More

Thank you for your enquiry.

Sorry for the confusion here. A large excess of Standard is provided in this ELISA kit. To properly dilute the standard, just reconstitute the 3.5ug Standard you received in 0.7ml of Diluent to produce a 5ug/ml Sta...

Read More

Abreview for Hemopexin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum
Amount of Sample: 2 ul µg
Type of Kit Used: ELISA
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: We diluted 2ul of plasma with 800 ul of Diluent (1:400).
We washed the plate 6 times with a machine.
The standards data generated an excelent curve with a fitting error of
3.48e-5.
All samples assayed showed data inside the curve.
Username

Dr. Rafael Montes Nieto

Verified customer

投稿 Mar 26 2012

Thank you for your phone call. Please find testing data for ab108859 attached. I hope this helps, please let me know if you need any additional information or assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"