Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab108861)

製品の概要

  • 製品名Human Heat Shock Protein 47 ELISA Kit (SERPINH1)
    Hsp 47 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 2.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.6%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Milk, Serum, Plasma, Tissue, Cell Lysate
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    > 0.9 ng/ml
  • 検出範囲
    1.563 ng/ml - 50 ng/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Heat Shock Protein 47 (SERPINH1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP47 levels in Human cell culture lysates, milk, serum, plasma and tissue extract.

    A HSP47 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP47 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP47 captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 関連疾患Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10) [MIM:613848]. A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae.
  • 配列類似性Belongs to the serpin family.
  • 細胞内局在Endoplasmic reticulum lumen.
  • Information by UniProt
  • 別名
    • 47 kDa heat shock protein
    • Arsenic-transactivated protein 3
    • AsTP3
    • Cell proliferation-inducing gene 14 protein
    • Collagen-binding protein
    • Collagen-binding protein 1
    • Colligin
    • Rheumatoid arthritis-related antigen RA-A47
    • SERPH_HUMAN
    • Serpin H1
    • Serpin Peptidase Inhibitor
    • SERPINH1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Heat Shock Protein 47 ELISA Kit (SERPINH1) 画像

  • Representative Standard Curve using ab108861.

プロトコール

Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab108861) 使用論文

This product has been referenced in:
  • Fang JY  et al. Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics. Proteomics 16:2718-2731 (2016). Read more (PubMed: 27459910) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"