Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab113334)

製品の概要

  • 製品名Human Heat Shock Protein 27 ELISA Kit (HSP27)
    Heat Shock Protein 27 (HSP27) キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.15 ng/ml
  • 検出範囲
    0.205 ng/ml - 50 ng/ml
  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 133.1 123% - 151%
    Serum 126.9 117% - 134%
    Plasma 119.8 105% - 142%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Heat Shock Protein 27 (HSP27) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HSP27 in serum, plasma, cell culture supernatants.


    This assay employs an antibody specific for Human Heat Shock Protein 27 coated on a 96-well plate. Standards and samples are pipetted into the wells and Heat Shock Protein 27 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Heat Shock Protein 27 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Heat Shock Protein 27 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    ave time with a 90-minute ELISA protocol. Try Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab193757) from our SimpleStep ELISA® range.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Involved in stress resistance and actin organization. Interacts with TGFB1I1. Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 and HSPBAP1.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasm; cytoskeleton; spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • 別名
    • HSPB1
    • 28 kDa heat shock protein
    • CMT2F
    • estrogen regulated 24 kDa protein
    • Estrogen-regulated 24 kDa protein
    • Heat shock 27 kDa protein
    • heat shock 27kDa protein 1
    • heat shock protein beta 1
    • Heat shock protein beta-1
    • HMN2B
    • HS.76067
    • HSP 27
    • Hsp25
    • HSP27
    • HSP28
    • HspB1
    • SRP27
    • stress responsive protein 27
    • Stress-responsive protein 27
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Heat Shock Protein 27 ELISA Kit (HSP27) 画像

  • Representative Standard Curve using ab113334

プロトコール

Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab113334) 使用論文

ab113334 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"