製品の概要

  • 製品名Human Heat Shock Protein 27 ELISA Kit (HSP27)
    Heat Shock Protein 27 (HSP27) キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.3%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Milk, Serum, Plasma, Cell culture extracts, Tissue
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    > 0.3 ng/ml
  • 検出範囲
    0.313 ng/ml - 80 ng/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Heat Shock Protein 27 (HSP27) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP27 levels in cell culture samples, milk, serum, plasma and tissue extract.

    A HSP27 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP27 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP27 captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Involved in stress resistance and actin organization. Interacts with TGFB1I1. Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 and HSPBAP1.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasm; cytoskeleton; spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • 別名
    • HSPB1
    • 28 kDa heat shock protein
    • CMT2F
    • estrogen regulated 24 kDa protein
    • Estrogen-regulated 24 kDa protein
    • Heat shock 27 kDa protein
    • heat shock 27kDa protein 1
    • heat shock protein beta 1
    • Heat shock protein beta-1
    • HMN2B
    • HS.76067
    • HSP 27
    • Hsp25
    • HSP27
    • HSP28
    • HspB1
    • SRP27
    • stress responsive protein 27
    • Stress-responsive protein 27
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Heat Shock Protein 27 ELISA Kit (HSP27) 画像

  • Representative Standard Curve using ab108862.

プロトコール

Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab108862) 使用論文

This product has been referenced in:
  • White NM  et al. Quantitative proteomic analysis reveals potential diagnostic markers and pathways involved in pathogenesis of renal cell carcinoma. Oncotarget 5:506-18 (2014). Read more (PubMed: 24504108) »
  • Enomoto M  et al. Newborn rat response to single vs. combined cGMP-dependent pulmonary vasodilators. Am J Physiol Lung Cell Mol Physiol 306:L207-15 (2014). WB . Read more (PubMed: 24242011) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"