Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab108862)
Key features and details
- Sensitivity: 0.16 ng/ml
- Range: 1.25 ng/ml - 20 ng/ml
- Sample type: Cell Lysate, Milk, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Heat Shock Protein 27 ELISA Kit (HSP27)
Heat Shock Protein 27 (HSP27) キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 9.9% -
サンプルの種類
Milk, Serum, Plasma, Tissue, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
> 0.16 ng/ml -
検出範囲
1.25 ng/ml - 20 ng/ml -
添加回収試験
97 %
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全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Heat Shock Protein 27 (HSP27) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP27 levels in cell culture samples, milk, serum, plasma and tissue extract.
A HSP27 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP27 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP27 captured in plate.
Get results in 90 minutes with Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab193757) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human HSP27 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml HSP27 Microplate (12 x 8 well strips) 1 unit HSP27 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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関連性
Involved in stress resistance and actin organization. Interacts with TGFB1I1. Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 and HSPBAP1. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasm; cytoskeleton; spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. -
別名
- HSPB1
- 28 kDa heat shock protein
- CMT2F
see all -
参照データベース
- Entrez Gene: 3315 Human
- Omim: 602195 Human
- SwissProt: P04792 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab108862 は 5 報の論文で使用されています。
- Zhou XY et al. TAT-HSP27 Peptide Improves Neurologic Deficits via Reducing Apoptosis After Experimental Subarachnoid Hemorrhage. Front Cell Neurosci 16:878673 (2022). PubMed: 35573833
- White NM et al. Quantitative proteomic analysis reveals potential diagnostic markers and pathways involved in pathogenesis of renal cell carcinoma. Oncotarget 5:506-18 (2014). PubMed: 24504108
- Enomoto M et al. Newborn rat response to single vs. combined cGMP-dependent pulmonary vasodilators. Am J Physiol Lung Cell Mol Physiol 306:L207-15 (2014). WB . PubMed: 24242011
- Jin C et al. Plasma heat shock protein 27 is associated with coronary artery disease, abdominal aortic aneurysm and peripheral artery disease. Springerplus 3:635 (2014). PubMed: 25392804
- Thanabalasundaram G et al. Cortical non-aneurysmal subarachnoid hemorrhage post-carotid endarterectomy: a case report and literature review. Springerplus 2:571 (2013). PubMed: 24255864