製品の概要

  • 製品名
    Human HDL ELISA Kit
    HDL キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.2%
  • サンプルの種類
    Cell culture supernatant, Milk, Serum, Plasma
  • アッセイタイプ
    Competitive
  • 検出感度
    6 µg/ml
  • 検出範囲
    6.25 µg/ml - 400 µg/ml
  • 添加回収試験

    96.5 %

  • 全工程の試験時間
    3h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s High-Density Lipoprotein (HDL) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human HDL in plasma, serum, milk, and cell culture supernatant.

    A HDL specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated HDL is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of HDL captured in plate.

  • アプリケーション
    適用あり: Competitive ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

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  • Representative Standard Curve using ab125961

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参考文献

This product has been referenced in:
  • Ito F  et al. The Application of a Modified d-ROMs Test for Measurement of Oxidative Stress and Oxidized High-Density Lipoprotein. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28230785) »

See 1 Publication for this product

レビューと Q&A

The ab125961 Human HDL ELISA Kit antibodies are polyclonal antibody against total HDL and recognize lipoprotein in the HDL.  It has some cross reactivity with Lp(a).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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