製品の概要

  • 製品名Human HB EGF ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 20 pg/ml
  • 検出範囲
    16.38 pg/ml - 4000 pg/ml
  • 添加回収試験

    90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 81.22 73% - 90%
    Serum 99.18 89% - 108%
    Plasma 93.73 84% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s HB EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HB EGF in serum, plasma, and cell culture supernatants. (Human HB EGF concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human HB EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HB EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HB EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HB EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human HB EGF ELISA Kit 画像

  • Representative standard curve using ab100531

  • Representative standard curve using ab100531

プロトコール

Human HB EGF ELISA Kit (ab100531) 使用論文

ab100531 has not yet been referenced specifically in any publications.

Product Wall



1. Increase amount of biotinylated ab (by 1.5 fold or so – too much may increase background)
2. Incubate biotinylated ab overnight at 4 degrees C
3. Increase amount of HRP-strep (by about 1.5 fold or so – to...

Read More

Thank you for your interest in our ELISA kits. Each kit comes with a microplate that is already coated with an antibody specific for the the protein of interest, so you would not be able to use one plate to detect all three proteins (EGF, TGF, betacell...

Read More

Thank you for contacting us.

I am very sorry to hear these kits failed to give the expected results. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to ...

Read More

Thank you for the updates.

We rarely recommend running plasma or serum samples undiluted, as matrix effects are more likely to occur. For this kit (ab100531: HB EGF Human ELISA Kit), we recommend a 2-fold dilution of plasma or serum. This may...

Read More

Muchas gracias por contactarnos. Le pido disculpas por la espera.

El kit al que se refiere es valido para detectar HB EGF humano en suero, plasma y sobrenadante de cultivo celular. En el protocolo adjunto en la página web se indican lo...

Read More

Thank you for the enquiry regarding ab100531 and for sending the customer’s data/comments.

Please find enclosed the attached file for the recent analysis. As you can see, the standard curves obtained have good linearity and very low CV%...

Read More

Thank you for your enquiry regarding ab100531. I am very sorry to hear that your customer having problems with this kit.

The batch number you have provided (81802) does not match to our record. Could you please check it again and confirm the ...

Read More

Thank you for your call yesterday and for your questions. I have been in touch with my colleague in the lab, and have received more information about ab100531.

The standard provided in ab100531 is expressed in baculovirus, and it corresonds t...

Read More

Thank you for contacting Abcam.

Youshould use Assay Diluent C if diluting conditioned media samples.

Please letme knowif you haveany questions.

Thank you for contacting Abcam.

Please find the answers to your questions below:

1. are the coating antibody and the detection antibody a polyclonal or
monoclonal antibodies?Capture antibody = monoclonal
Detection antibody...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"