製品の概要

  • 製品名Human Haptoglobin ELISA Kit
    Haptoglobin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.2%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプCompetitive
  • 検出感度
    = 0.07 µg/ml
  • 検出範囲
    0.078 µg/ml - 20 µg/ml
  • 添加回収試験

    = 98 %

  • 全工程の試験時間
    2h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Haptoglobin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin in plasma and serum.

    A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Haptoglobin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Haptoglobin captured in plate.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Defects in HP are the cause of anhaptoglobinemia (AHP) [MIM:614081]. AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • binding peptide
    • BP
    • haptoglobin alpha 1S beta
    • haptoglobin alpha 2FS beta
    • haptoglobin alpha polypeptide
    • Haptoglobin beta chain
    • haptoglobin beta polypeptide
    • HP
    • HP2ALPHA2
    • HPA1S
    • HPT_HUMAN
    • Zonulin
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent dilution.

Human Haptoglobin ELISA Kit 画像

  • Haptoglobin measured in various biological fluids showing quantity (μg) per mL of sample tested

  • Representative Standard Curve using ab108856.

プロトコール

Human Haptoglobin ELISA Kit (ab108856) 使用論文

This product has been referenced in:
  • Chang G  et al. Feeding a high-grain diet reduces the percentage of LPS clearance and enhances immune gene expression in goat liver. BMC Vet Res 11:67 (2015). Read more (PubMed: 25889631) »

See 1 Publication for this product

Product Wall

You do not need to subtract a blank for competitive ELISA. If you do, you will end up with negative values.

Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2)...

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Thank you for your inquiry.

I heard back from the lab that serum that has undergone hemolysis will have a small interference with the haptoglobulin, but you should be able to use the sample. You should do a background control in this case. Read More

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.
The Haptoglobulin Human ELISA Kits (ab108856 and ab108858) can be used in order to quantify the level of haptoglobulin in various biolo...

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Thank you very much for your interest in ab108856

To our knowledge, ab108856 has not been tested in Urine samples. However by participating in our AbTrial program you can now use our products in an untested application or species without fin...

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Thank you very much for your reply and for checking on the other 2 kits.

I apologize again for these errors. I looked through our records, and we haven't received any other reports of problems with ab108856, so I think that the quality of the...

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Thank you very much for your email and for letting us know about the trouble with this kit.

I've checked all of our stock notes on this kit, and I've examined 3 of the kits that we have in stock. The haptoglobin standard should have arrived l...

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Thank you very much for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

I have received the following information from the lab:

The proper value for Haptoglobulin in human pl...

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Te pido disculpas por la espera y agradezco tu paciencia.

Me confirman mis compañeros del laboratorio que ambos kits (ab108856 y ab108858) detectan la subunidad beta de la proteína. Además, si la sensibilidad del kit ab10...

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Thank you for your reply.

The standard curve you had sent me originally looked good. I assume these are the same data.

I apologize, I was mistaken.The blank has the highest value, thus you would not be able to subtract it from the ...

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