Human Granzyme B ELISPOT Kit (without plates) (ab46581)

製品の概要

  • 製品名
    Human Granzyme B ELISPOT Kit (without plates)
    Granzyme B キット 製品一覧
  • サンプルの種類
    Cell culture supernatant, Cell culture extracts
  • アッセイタイプ
    Sandwich (qualitative)
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    This Elispot assay is designed to enumerate Granzyme B producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing Granzyme B production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of Granzyme B producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

     

    Principal:

     

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

     

    Recognizes natural human Granzyme B

  • アプリケーション
    適用あり: ELISpotmore details
  • 試験プラットフォーム
    Reagents

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests
    Bovine Serum Albumin 1 x 1g 2 x 1g 3 x 1g 4 x 1g
    Dry Skimmed milk 1 x 1g 2 x 1g 3 x 1g 4 x 1g
    Biotinylated detection antibody (Lyophilized) 1 vial 2 vials 3 vials 4 vials
    Granzyme B Capture Antibody 1 x 500µl 2 x 500µl 3 x 500µl 4 x 500µl
    Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml 3 x 50ml 4 x 50ml
    Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl 3 x 50µl 4 x 50µl
  • 研究分野
  • 関連性
    Cytolytic T lymphocytes and natural killer cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
  • 細胞内局在
    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells
  • 別名
    • C11
    • CGL1
    • CSPB
    • CTLA1
    • Fragmentin 2
    • Granzyme 2
    • SECT
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISpot Use at an assay dependent dilution.

参考文献

ab46581 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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